Review





Similar Products

cd31 cells  (Miltenyi Biotec)
95
Miltenyi Biotec cd31 cells
(A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). <t>CD31+</t> EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)
Cd31 Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd31 cells/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
cd31 cells - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
cd31 antibody  (Miltenyi Biotec)
95
Miltenyi Biotec cd31 antibody
(A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). <t>CD31+</t> EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)
Cd31 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd31 antibody/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
cd31 antibody - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
αcd31 vioblue reafinitytm  (Miltenyi Biotec)
95
Miltenyi Biotec αcd31 vioblue reafinitytm
(A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). <t>CD31+</t> EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)
αcd31 Vioblue Reafinitytm, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/αcd31 vioblue reafinitytm/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
αcd31 vioblue reafinitytm - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
anti cd31 microbead kit miltenyi biotec  (Miltenyi Biotec)
97
Miltenyi Biotec anti cd31 microbead kit miltenyi biotec
(A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). <t>CD31+</t> EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)
Anti Cd31 Microbead Kit Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd31 microbead kit miltenyi biotec/product/Miltenyi Biotec
Average 97 stars, based on 1 article reviews
anti cd31 microbead kit miltenyi biotec - by Bioz Stars, 2026-03
97/100 stars
  Buy from Supplier
cd31 antibody, anti-human, reafinity  (Miltenyi Biotec)
94
Miltenyi Biotec cd31 antibody, anti-human, reafinity
(A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). <t>CD31+</t> EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)
Cd31 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd31 antibody, anti-human, reafinity/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
cd31 antibody, anti-human, reafinity - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
anti cd31  (Miltenyi Biotec)
99
Miltenyi Biotec anti cd31
(A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). <t>CD31+</t> EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)
Anti Cd31, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd31/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
anti cd31 - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
anti human cd31  (Proteintech)
96
Proteintech anti human cd31
(A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). <t>CD31+</t> EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)
Anti Human Cd31, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human cd31/product/Proteintech
Average 96 stars, based on 1 article reviews
anti human cd31 - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
anti-human cd3  (Bio-Rad)
94
Bio-Rad anti-human cd3
(A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). <t>CD31+</t> EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)
Anti Human Cd3, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-human cd3/product/Bio-Rad
Average 94 stars, based on 1 article reviews
anti-human cd3 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier

Image Search Results


(A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). CD31+ EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)

Journal: bioRxiv

Article Title: Forward Programming Identifies Inducers of Blood-Brain Barrier Properties in Human Pluripotent Stem Cell-Derived Endothelial Cells

doi: 10.64898/2026.02.02.699492

Figure Lengend Snippet: (A) IMR90-4 hPSCs were differentiated to endothelial progenitor cells (EPCs). CD31+ EPCs were MACS-sorted. Lentivirus for TF overexpression was dosed on CD31+ EPCs and the population was simultaneously treated with CHIR99021 to activate Wnt signaling. After five days, the resultant cell population is referred to as forward programmed CNS-like ECs (fpCECs). Bulk RNA sequencing was performed on the fpCECs at N=4 independent lentivirus transductions for each condition. (B) Principal component analysis (PCA) was performed on fpCECs and CECs overexpressing GFP as a control. A second PCA (right) was performed on samples within the dashed box in the first PCA (left) to better resolve the more closely related TF-driven transcriptomes. Each sample on the graphs represents an independently sequenced biological replicate. (C) Euclidean distance between the transcriptomes of fpCECs and the average transcriptome of CECs overexpressing GFP is plotted (see Methods for details). The mean±S.D. of N=4 sequencing replicates is plotted. (D) Heatmap of the expression of 88 BBB-enriched genes for fpCECs overexpressing each of the 21 candidate TFs. Each row represents z-scores normalized to the mean expression for each of the 88 BBB-enriched genes. The rightmost column is the GFP control. Average z-score of N=4 biological replicates is plotted. (E) BBB score analysis of fpCECs overexpressing each of the 21 candidate TFs. See the Methods for BBB score calculation details. The mean±S.D. of N=4 sequencing replicates is plotted. Colors for individual TFs in plots (C) and (E) match. (F) Heatmap of the expression of each of the 21 candidate TFs upon overexpression of each individual TF on the x-axis. Average Log 2 fold-changes compared to the GFP control are plotted for N=4 biological replicates. (G) String-style map for gene expression regulation amongst the 21 candidate TFs. For example, if TF A overexpression drives the upregulation of TF B more than two-fold in a statistically significant manner, an arrow from TF A to TF B is plotted. A p adj <0.05 using the Wald test followed by Benjamini-Hochberg correction was used as the statistical significance cutoff. TFs not represented on the string map did not have their expression affected (> 2-fold) by any other overexpressed TF. Full transcriptome expression data for all TFs and their replicates can be found in Supplementary File 1)

Article Snippet: On day 5, EPCs were purified by magnetic sorting of CD31+ cells, using anti-CD31-biotin antibody (Miltenyi 130-110-667), anti-biotin microbeads (Miltenyi 130-097-046) and a QuadroMACS separator (Miltenyi 130-091-051).

Techniques: Over Expression, RNA Sequencing, Control, Sequencing, Expressing, Gene Expression